云南农业大学学报2012，Vol.27Issue(5)：623-630,8.DOI:10.3969/j.issn.1004-390X(n).2012.05.002
小麦EST-SSR的分析及其引物的开发
Analysis and Exploitation of EST-SSRs in the Triticum aestivum
摘要
Abstract
A total of 1 071 367 expressed sequence tags (ESTs) of Triticum aestivum was searched for mono- to hexa-nucleotide simple sequence repeats ( SSR or microsatellite) using software of " Tandem Repeats Finder". And the type, size and frequency of these SSRs were determined. The results showed that a total of 32 350 SSRs were found out in these ESTs with the criteria of SSR length =24 bp and 80% matches. Considering all six classes of SSR, the most abundant microsatellite was mono-nucleotide repeat, and the number of mono-nucleotide repeat SSR was 18 075, occupied 55. 9% of total SSRs, followed by tri-nucleotide repeat, which number was 6 106, accounting for 18. 7% of all SSRs, and there were 444 tri-nucleotide repeat SSRs whose repeat number was more than 30, accounting for 7. 2%. The least microsatellites were tetra-nucleotide, their number was 696, only accounting for 2. 2% of all SSRs. Especially, 1 562 hexa-nucleotide repeats SSRs were observed, and their motifs were also very abundance, with 145 types, and AGCCGC is the major motif, being 214. In this study, because the size of all SSRs was more than or equal to 24 bp, these results would be useful for further molecular biological studies and development of new EST-SSR molecular markers of T. aeslivum.关键词
小麦/表达序列标签/微卫星Key words
wheat (Triticum aestivun) / expressed sequence tags (EST) / simple sequence repeat (SSR)分类
农业科技引用本文复制引用
刘林,孙来亮,兰茗清,杨静,查友贵,李成云..小麦EST-SSR的分析及其引物的开发[J].云南农业大学学报,2012,27(5):623-630,8.基金项目
公益性行业科研专项(200903035-07) （200903035-07）
云南省自然科学基金重点项目(2009CC004). （2009CC004）
