云南农业大学学报2012，Vol.27Issue(5)：687-692,6.DOI:10.3969/j.issn.1004-390X(n).2012.05.012
日粮能量水平对乌金猪脂肪代谢相关酶活性的影响
Effect of Dietary Energy Levels on Activity of Lipids Metabolic Enzymes in Wujin Pigs
摘要
Abstract
The aim of the study was to investigate the effects of dietary energy levels on lipids metabolic enzymes of liver and adipose tissue in Wujin pigs. Fifty-four Wujin pigs were randomly allotted into three groups fed diet containing different energy levels, there were eighteen pigs in each group with three replicates , and six pigs were used in per replicates. The dietary energy levels were high energy (HE, 14. 22 MJ/kg), medium energy (ME, 12. 89 MJ/kg) and low energy (LE, 11. 74 MJ/kg) respectively. At the body weight of 30, 60 and 100 kg, Wujin pigs were slaughtered and liver and back subcutaneous fatty tissue were taken for analyzing the activity of lipase ( DPS) , glucose-6 phosphate dehydrogenase (G-6-PDH) and malate dehydrogenase (MDH). The results showed that the activity of G-6-PDH and MDH in liver and back subcutaneous fatty tissue increased as Wujin pigs fed dietary of energy level increased, but, contents of UPS in liver and back subcutaneous fatty tissue decreased as Wujin pigs fed dietary of energy level increased, At 30kg body weight, the activity of LPS, G-6-PDH and MDH in liver and back subcutaneous fatty tissue of Wujin pigs were affected not significantly by the dietary energy level ( P > 0. 05 ). At 60 and 100 kg body weight, the LPS and G-6-PDH activity in liver and back subcutaneous fatty tissue of Wujin pigs fed on the high energy level dietary were significantly lower than the low energy level dietary (P <0. 05) , the MDH activity in liver and back subcutaneous fatty tissue of Wujin pigs fed on the high energy level dietary were significantly higher than the low energy level dietary (P < 0. 05 ). The results indicated that higher energy level of dietary could decrease endogenous energy release, increase exogenous energy availability and promote fat deposition in Wujin pigs at growth midanaphase.关键词
乌金猪/日粮能量水平/脂肪代谢/酶Key words
Wujin pigs/ dietary energy level/ fat metabolism/ enzyme分类
农业科技引用本文复制引用
潘洪彬,陈强,黄英,张永云,赵素梅,高士争..日粮能量水平对乌金猪脂肪代谢相关酶活性的影响[J].云南农业大学学报,2012,27(5):687-692,6.基金项目
云南省自然科学基金重点项目(2005C0008Z). （2005C0008Z）
