云南农业大学学报2012，Vol.27Issue(5)：703-707,5.DOI:10.3969/j.issn.1004-390X(n).2012.05.015
两种铁皮石斛总RNA提取方法的比较研究
Comparison on Two Modified Total RNA Isolating Methods from Dendrobium officinale
摘要
Abstract
Dendrobium qfficinale Kimura et Migor is an important Chinese medicinal material, in which polysaccharide and polyphenol is rich, however, it is difficult to isolate RNA from its leaf. We modified both the GTC method and CTAB method. Guanidine thiocyanate method: PVP was added to the GTC buffer to remove polyphenol; the plant tissue was decomposed quickly with 56℃ heated water; the extracting time was shortened from 15min to 5min to decrease the loss of RNA; regular doze of GTC buffer was wisely added according to the the amount of polysaccharide and polyphenol. CTAB method; adequate doze of CTAB buffer was added according to the amount of polysaccharide and polyphenol of the extracting material; the RNA was precipitated overnight with LiCl, which RNA but not DNA could be precipitated, and a certain doze of LiCl was added wisely would be better. The result showed that RNA could be extracted by the two modified GTC method and CTAB method. Moreover, it is evident that the latter is more suitable for biomolecular research, of which RNA is purer and intact.关键词
铁皮石斛/多糖/总RNA提取/异硫氰酸胍法/CTAB法Key words
Dendrobium qfficinale/ polysaccharide/ RNA isolation/ GTC method/ CTAB method分类
农业科技引用本文复制引用
容天聚,文国松,钱雄,查应洪,徐绍忠..两种铁皮石斛总RNA提取方法的比较研究[J].云南农业大学学报,2012,27(5):703-707,5.基金项目
云南省重大产业项目资助项目[云发改高技(2007)1718号] （2007）
国家支撑计划资助项目(2011 BAI13B02-5). （2011 BAI13B02-5）
