医药导报2012，Vol.31Issue(9)：1200-1202,3.DOI:10.3870/yydb.2012.09.031
不同地域东亚钳蝎蝎毒的双向电泳鉴定
Identification of Scorpion Venom in Buthus Martensii Karsch from Different Regions by Two-dimensional Gel Electrophoresis (2-DE)
倪伊婷 1郭美华 1王君德 2刘赟 3辛毅 1吴大畅1
作者信息
- 1. 大连医科大学生物技术系,116044
- 2. 大连医科大学国有资产管理处,116044
- 3. 天津医科大学,300070
- 折叠
摘要
Abstract
Objective To identify qualitatively and analyze the activity of scorpion venom in Buthus Martensii Karsch from different regions by two-dimensional gel electrophoresis (2-DE),and investigate the protein composition and function differences of scorpion venom. Methods Quantitive identification and content determination of proteins in scorpion venom were performed following preconditions including dissolution, desalting and condensing of the freeze-drying scorpion venom powder. The proteins in scorpion venom were separated by pH gradient isoeleciric focusing and SUS-PAGE gel eledrophoresis. The two-dimensional electrophoresis gel map was captured via gel imaging system after staining. The special different proteins were determined and comparatively analyzed through PD Quesl. Image analysis software,and thus the scorpion venom from different regions was qualitatively identified. Results Protein fingerprints were acquired from three samples. Total protein spots were 80,69,77,and the distinctive number of which were 56,46,55 , successively. Conclusion Scorpion venom in Buthus Martensii Karsch from diffe.re.nt regions separated with 2-DE show obviously diverse protein distributions.关键词
蝎毒/东亚钳蝎/双向电泳/鉴定Key words
Scorpion venom/ Buthus Martemii Karsch/ Two-dimensional gel electrophoresis/ Identification分类
医药卫生引用本文复制引用
倪伊婷,郭美华,王君德,刘赟,辛毅,吴大畅..不同地域东亚钳蝎蝎毒的双向电泳鉴定[J].医药导报,2012,31(9):1200-1202,3.
