中国病理生理杂志2012，Vol.28Issue(10)：1766-1772,7.DOI:10.3969/j.issn.1000-4718.2012.10.007
TGF-β1/Smads和ERK表达异常在高盐饮食诱导的大鼠血管重构中的作用
Role of TGF-β1/Smads and ERK expression in vascular remodeling induced by high-salt diet in Wistar rats
摘要
Abstract
AIM; To investigate the role of transforming growth factor β1 (TGF -β1 )/Smads and extracellular signal - regulated kinase( ERK) expression in vascular remodeling induced by high - salt diet in Wistar rats. METHODS: Wistar rats were randomly divided into 3 groups: normal control group ( n = 13 ) , high salt ( 8 % ) model group and high salt + telmisartan group (n = 13). Tail - cuff arterial pressure was determined every 2 weeks. After 24 weeks, the rats in high salt model group were divided into model animals with hypertension group (MH, n = 12) and model animals without hypertension group ( MN, n = 12). The remodeling of aorta and mesenteric artery was observed by HE and Masson stai-ning. In addition, the techniques of immunohistochemistry and real - time PCR were applied to detect the expression of proliferating cell nuclear antigen ( PCNA) , TGF -β1 , p - Smad2/3, p - ERK1/2 and Smad7 at both protein and mRNA levels. RESULTS: Compared with normal control group, blood pressure in MH group was much higher, and media thick-ness ( MT) and collagen volume fraction ( CVF) of arteries in MH and MN groups were higher. The mRNA expression of TGF - β1 , Smad2 and Smad7 in the aorta was significantly increased, and the protein levels of PCNA, p - ERK1/2, TGF -β1 and p - Smad2/3 in the aorta and mesenteric artery media were elevated, but Smad7 decreased. After telmisartan treatment, MT and CVF were much lower,and the protein levels of PCNA, TGF - β1 , p - Smad2/3 and p - ERK1/2 were significantly reduced, whereas Smad7 was increased. CONCLUSION: The abnormal expression of TGF - β1/Smads and ERK may be involved in the mechanism of remodeling of aorta and mesenteric artery induced by high - salt diet. Telmisar- tan prevents the vascular remodeling via regulating TGF - β1/Smads and ERK signal pathways mediated by angiotensin Ⅱ type 1 ( AT1 ) receptor, at least in part.关键词
高盐饮食/血管重构/转化生长因子β1/Smad蛋白类/细胞外信号调节激酶Key words
High - salt diet/Vascular remodeling/Transforming growth factor β1 /Smad proteins/Extracel-lular regulated protein kinase分类
医药卫生引用本文复制引用
刘婵,商黔惠,闵晓强,陈剑玲,毛万姮..TGF-β1/Smads和ERK表达异常在高盐饮食诱导的大鼠血管重构中的作用[J].中国病理生理杂志,2012,28(10):1766-1772,7.基金项目
国家自然科学基金资助项目(No.81160041) （No.81160041）
贵州省社会发展攻关计划项目和省高层次人才科研条件特助项目[No.黔科合SY字(2011)3047号 （2011）
TZJF-2009年-42] （）
