中国病理生理杂志2012，Vol.28Issue(10)：1802-1806,5.DOI:10.3969/j.issn.1000-4718.2012.10.013
黄芪注射液联合葛根素注射液对2型糖尿病KKAy 小鼠肾脏TGF-β1和BMP-7表达的影响
Effects of Astragalus injection combined with puerarin injection on expression of BMP-7 and TGF-β1 in type 2 diabetic KKAy mouse kidney
摘要
Abstract
AIM; To observe the effects of Astragalus injection combined with puerarin injection on the expres-sion of transforming growth factor beta 1 ( TGF - β1 ) and bone morphogenetic protein 7 ( BMP - 7 ) in the kidney of type 2 diabetic KKAy mouse. METHODS: The male KKAy mice of 14 weeks old were randomly divided into model group and Astragalus injection combined with puerarin injection treatment (Astragalus + puerarin) group. The age-matched male C57BL/6J mice were selected as normal group. The general conditions and body weight of the mice were observed. Blood glucose ( BG) , triglyceride (TG) , cholesterol (TC) and serum creatinine (SCr) were examined at the 20th, 24th and 28th week. The protein expression of renal TGF - β1 was determined by immunohistochemical method. The mRNA expres-sion of BMP -7 and TGF - β1 was detected by RT - PCR. RESULTS: Compared with normal group, the body weight, BG, TG, TC and SCr increased significantly in model group. TGF - β1 expression at protein and mRNA levels was in-creased, while mRNA expression of BMP -7 was decreased in KKAy mice. Compared with model group, the body weight, BG, TG, TC and SCr reduced in Astragalus + puerarin group. The mRNA expression of BMP -7 in the renal tissues was higher, and TGF - β1 expression at mRNA and protein levels was significantly lower in Astragalus + puerarin group than those in model group. CONCLUSION: Astragalus injection combined with puerarin injection has renal protective effects on type 2 diabetic KKAy mice. The mechanism may be related to restoring BMP - 7 expression and reducing the overexpres-sion of TGF - β1 in renal tissues.关键词
黄芪注射液/葛根素注射液/糖尿病肾病/转化生长因子-β1/骨形态发生蛋白-7Key words
Astragalus injection/Puerarin injection/Diabetic nephropathies/Transforming growth factor β1 /Bone morphogenetic protein 7分类
医药卫生引用本文复制引用
李姝玉,柴新楼,吴莹,贾德贤,郝钰,苏玮莲,王谦..黄芪注射液联合葛根素注射液对2型糖尿病KKAy 小鼠肾脏TGF-β1和BMP-7表达的影响[J].中国病理生理杂志,2012,28(10):1802-1806,5.基金项目
国家自然科学基金资助项目(No.30672756 （）
No.81072926) （）
北京中医药大学创新团队项目(No.2011-CXD-04) （No.2011-CXD-04）
