中国工程科学2012，Vol.14Issue(8)：85-90,6.
胶质瘤细胞系摄取BPA的实验研究
Experimental study on BPA uptake of glioma cell lines
摘要
Abstract
Objective: to evaluate the incorporation of BPA by glioma cell lines, and to observe its relationship with the temperature and the concentration of BPA. Methods; C6, U251 and rat astrocyte cells were incubated in a culture medium, in which ' B concentration was 20, 40, 60, 80, 100 μg/mL for 24 h. Boron concentration in the cells was measured induced couple plasma-atomic emission spectroscopy (ICP-AES). C6 cells were pre-incu-bated for 24 h with different boron concentrations in growth medium. Then the mediums were changed to boron-free ones, and boron content was assessed after 1, 2, 3 h. Results; The content of the 10B in cells was increasing with the increasing concentration of BPA, and the boron concentration ratios of glioma cells to astrocyte are 2. 2. The BPA efflux is slower at the lower temperature. Conclusion; BPA has a selectivity for glioma cells, and the results of the efflux assay confirm the temperature dependence of the BPA transport out of the cells.关键词
硼中子俘获疗法/BPA/胶质瘤细胞系/感应耦合等离子体原子发射光谱Key words
boron neutron capture therapy/BPA/glioma cell line/ICP-AES分类
医药卫生引用本文复制引用
杨磊,赵葵,王潇,隋丽,孔福全,郝小娟,郑洁莹,马南茹,崔素珍,刘权卫..胶质瘤细胞系摄取BPA的实验研究[J].中国工程科学,2012,14(8):85-90,6.基金项目
国家自然科学基金项目(11079054) （11079054）
