中国骨伤2012，Vol.25Issue(8)：662-666,5.DOI:10.3969/j.issn.1003-0034.2012.08.012
骨水泥表面粗糙度对诱导膜组织学特性的影响
Effects of surface roughness of bone cements on histological characteristics of induced membranes
摘要
Abstract
Objective: To explore surface roughness of bone cement and surround tissue on histological characteristic of induced membranes. Methods: Bone cements with smooth and rough surface were implanted in radius bone defect, intramuscular and subcutaneous sites of rabbits, and formed induced membranes. Membranes were obtained and stained (HE) 6 weeks later. Images of membrane tissue were obtained and analyzed with an automated image analysis system. Five histological parameters of membranes were measured with thickness,area,cell density ,ECM density and microvessel density. Double factor variance analysis was used to evaluate the effect of the two factors on histological characteristics of induced membranes. Results : Membranes can be induced by each kind of bone cement and at all the three tissue sites. In histological parameters of thickness,area and micro vessel,there were significant differences among the membranes induced at different tissue sites (P= 0.000,P=0.000,P=0.000) ;whereas,there were no significant differences in histological parameters of cell density and ECM density(P=0.734,P=0.638). In all five histological parameters of membranes,there were no significant differences between the membranes induced by bone cements with different surface roughness (P=0.506,P=0.185 ,P=0.883,P=0.093,P=0.918).Conclusion: Surround tissue rather than surface roughness of bone cements can affect the histological characteristics of induced membranes. The fibrocystic number, vascularity, mechanical tension and micro motion of the surround tissue may be closely correlated with the histological characteristics of induced membranes.关键词
皮下组织/肌肉/骨缺损/骨水泥/组织学Key words
Subcutaneous tissue/ Muscles/ Bone defect/ Bone cement/ Histology引用本文复制引用
柳海晓,徐华梓,张宇,胡刚,沈跃,程小杰,彭磊..骨水泥表面粗糙度对诱导膜组织学特性的影响[J].中国骨伤,2012,25(8):662-666,5.基金项目
国家自然科学基金项目(面上项目)(编号:31060135/C100302) （面上项目）
浙江省医药卫生科学研究基金(编号:2007B147) （编号:2007B147）
