首页|期刊导航|中国海洋湖沼学报（英文版）|Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase
中国海洋湖沼学报（英文版）2012，Vol.30Issue(5)：724-730,7.
Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase
Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase
摘要
Abstract
Meretrix meretrix Linnaeus has long been used as traditional Chinese medicine in oriental medicine.The angiogentic activity of the extract of M.meretrix was investigated in this study,using human umbilical vein endothelial cells(HUVECs),Extract of M.meretrix Linnaeus(AFG-25)was prepared with acetone and ethanol precipitation,and further separated by Sephadex G-25 column.The results show that AFG-25 promoted proliferation,migration,and capillary-like tube formation in HUVECs,and in the presence of eNOS inhibitor NMA,the tube formation induced by AFG-25 is inhibited significantly.Moreover,AFG25 could also promote the activation of endothelial nitric oxide synthase(eNOS)and the resultant elevation of nitric oxide(NO)production.The results suggested that M.meretrix contains active ingredients with angiogentic activity and eNOS/NO signal pathway is in part involved in the proangiogenesis effect induced by AFG-25.关键词
Meretrix meretrix Linnaeus/angiogenesis/NOS/nitric oxide(NO)Key words
Meretrix meretrix Linnaeus/angiogenesis/NOS/nitric oxide(NO)引用本文复制引用
LIU Ming,WEI Jianteng,WANG Hui,DING Lili,ZHANG Yuyan,LIN Xiukun..Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase[J].中国海洋湖沼学报（英文版）,2012,30(5):724-730,7.基金项目
Supported by the Innovative Drug Development Projects of China(Nos.2009ZX09103-661 and 2009ZX09102)and the National Natural Science Foundation of China(No.81001396) （Nos.2009ZX09103-661 and 2009ZX09102）
