首页|期刊导航|中国海洋湖沼学报（英文版）|A new simple screening method for the detection of paralytic shellfish poisoning toxins
中国海洋湖沼学报（英文版）2012，Vol.30Issue(5)：786-790,5.
A new simple screening method for the detection of paralytic shellfish poisoning toxins
A new simple screening method for the detection of paralytic shellfish poisoning toxins
摘要
Abstract
The current testing for paralytic shellfish poisoning(PSP)in shellfish is based on the mouse bioassay(MBA).To alleviate animal welfare concerns,we evaluated the utility of using sublethal indicators of toxicity as an alternative to measuring time to death.Live mice were injected with a PSP congener and the changes in neurotransmitter levels were measured 60,90,and 120 min after injection.Acetylcholine (ACh)was the most sensitive marker for PSP toxicity.The changes in neurotransmitter levels were most pronounced in the blood.Thus,measurement of Ach levels in the blood may serve as a sensitive predictor for PSP that would not require sacrifice of the mice.This method was relatively simple,sensitive(less than 1 μg/kg weight,equivalent to 20 ng/mL),low maintenance,and rapid(less than 60 min).关键词
paralytic shellfish poisoning(PSP)/mouse bioassay/acetylcholine/assayKey words
paralytic shellfish poisoning(PSP)/mouse bioassay/acetylcholine/assay引用本文复制引用
CHENG Jinping,PI Shuaishuai,YE Shufeng,GAO Haomin,YAO Lei,JIANG Zhenyi,SONG Yuling,XI Lei..A new simple screening method for the detection of paralytic shellfish poisoning toxins[J].中国海洋湖沼学报（英文版）,2012,30(5):786-790,5.基金项目
Supported by the National High Techonlogy Research and Development Program of China (863 Program)(No.2007AA092001-15),the Key Laboratory of Marine Integrated Monitoring and Applied Technologies of Harmful Algal Blooms,Chinese State Oceanic Administration(No.MATHAB20120101),and the Shanghai Municipal Oceanic Bureau(Nos.2011-02,2012-02) （863 Program）
