农学学报2012，Vol.2Issue(9)：51-55,5.
不同酶解法水解豆粕蛋白的比较研究
Comparative Study of Soybean Protein Hydrolysates Prepared in Different Ways
摘要
Abstract
Comparative Studies on the effect of hydrolysis processes and products ot soybean proLem by different enzymic hydrolysis. In the present study, Alcalase protease and Protex.7L protease were utilized to hydrolyze soybean protein in different ways, such as single-enzyme hydrolysis and one-step and stepwise double-enzyme hydrolysis. After degree of hydrolysis, protein utilization, peptides yield, oligopeptides yield were measured to comparatively study soybean protein enzymolysis in different hydrolysis way. It was shown that the optimum hydrolysis way was sequential hydrolysis with Alcalase protease followed by Protex.7L protease. Under this way, the degree of hydrolysis, protein utilization, peptides yield, oligopeptides yield reached 23.5%, 68.7%, 51.0%, 21.4%. The stepwise double-enzyme hydrolysis was superior to single-enzyme hydrolysis and one-step double-enzyme hydrolysis, and that there was obvious relation between hydrolysis process of soybean protein and the order of adding enzymes.关键词
豆粕/蛋白/酶水解/双酶水解Key words
Soybean/Protein/Enzymic Hydrolysis/Double-enzyme Hydrolysis分类
农业科技引用本文复制引用
宋永康,黄薇,林虬,姚清华,林香信..不同酶解法水解豆粕蛋白的比较研究[J].农学学报,2012,2(9):51-55,5.基金项目
福建省科技重大专项“利用生物技术进行饲料原料预处理的研究及应用” （）
福建省农科院双百项目“寡肽蛋白在水产饲料中应用技术示范推广”（sbmd1226）. （）
