中国农业大学学报2012，Vol.17Issue(4)：118-122,5.
利用体细胞核移植技术生产优秀种公猪
High performance boar cloned from somatic cell nuclear transfer
摘要
Abstract
To investigate the feasibility of the cloned high performance breeding boar by the technology of somatic cell nuclear transfer in actual production. The nuclear donors were selected from boars, ear organization cells, and the nuclear receptors come from some oecytes through in-vitro maturation. There are 3 437 pieces of 1 - 4 cells cloned embryos were transplanted to 18 receptors successively. Sixteen receptors were pregnancy （88.89%） at 28 d by B ultrasonic detection, and 53 piglets were obtained from 11 pregnancy receptors （68. 75%）. The cloned production efficiency was 2.80% （birth piglet/embryos）. The determination results of growth characteristics showed that there is no significant difference in the measurement of cloned bedyweight and length （P〉0.05） from ordinary boars at 0 and 7 d. Therefore,the cloned boars from the technology of somatic cell nuclear transfer can extend the space and span of a boar and enlarge the use value of the outstanding boar.关键词
种公猪/体细胞核移植/胚胎移植/克隆Key words
boar/somatic cell nuclear transfer/embryo transfer/clone分类
农业科技引用本文复制引用
薛振华,潘登科,朱振营,罗学明,云鹏,王晓凤,肖炜..利用体细胞核移植技术生产优秀种公猪[J].中国农业大学学报,2012,17(4):118-122,5.基金项目
北京市农业科技项目 （）
北京市优秀人才资助项目 （）
生猪产业技术体系北京市创新团队 （）
