中国农业大学学报2012，Vol.17Issue(5)：15-20,6.
alfAFP和spCEMA融合基因表达载体的构建及其对小麦的遗传转化
Co-transformation of wheat with fusion genes alfAFP and spCEMA using biolistics
摘要
Abstract
Transgenic wheat variety XiNong 1376 containing the plant defensin gene alfAFP and antimicrobial peptide gene spCEMA fusion gene were obtained using cotransformation with bar as a selective marker via biolistics.In total,269 plantlets were generated through screening,and 188 of which were alive after vernalization.Leaf genome DNA PCR array showed that 7 co-transformed plants were obtained,and the frequency of co-integration was 0.1%.Studies also illustrated that the shortened time of the bar gene screening process did not significantly reduce the efficiency of PPT screening.关键词
小麦/转基因/融合基因/共转化Key words
wheat/transgenic/gene fusion/co-transformation分类
农业科技引用本文复制引用
张立,王建锋,王晓杰,康振生,韩德俊..alfAFP和spCEMA融合基因表达载体的构建及其对小麦的遗传转化[J].中国农业大学学报,2012,17(5):15-20,6.基金项目
国家重大专项 （）
高等学校学科创新引智计划资助项目 （）
