中国农业大学学报2012，Vol.17Issue(5)：39-45,7.
草莓‘甜查理’和‘章姬’花药培养及单倍体植株的获得
Regenerating haploid callus and plantlets from anther culture of strawberry ’Sweet Charlie’ and ’Akihime’
摘要
Abstract
This research was to study the suitable conditions for anther culture of strawberry(Fragaria ananassa Duch.).The investigated impacts included the effects of different cold pretreatments,inflorescences and hormone concentrations on callus induction and differentiation in anther culture of strawberry.The results indicated that maintenance of moist surface of bud could improve the callus induction rate of anther culture during the cold pretreatment at 4 ℃.The most appropriate duration was 72 hours.For the cultivation of over-wintering strawberry in Beijing,the first and the second inflorescences were more suitable for anther culture than the third and the fourth on callus induction and differentiation.The suitable medium for callus induction was obtained.The callus induction rates of ’Sweet Charlie’ and ’Akihime’ were 91.67% and 87.68%,respectively.The best medium for differentiation was found.The differentiation rates of ’Sweet Charlie’ and ’Akihime’ were 19.10% and 10.59%.Totally,688(including 445 ’Sweet Charlie’ and 243 ’Akihime’ plants) anther derived plants were obtained through this experiment.In the analysis of ’Sweet Charlie’,diploids covered(2n=8x=56) 90.43%,haploids(2n=4x=28) 7.62% and the others were aneuploids among the 445 regenerated plants.For ’Akihime’,diploids covered 93.42%,haploids 4.32% and the others were aneuploids among the 243 regenerated plants.A high frequency induction system of strawberry was established by anther culture and haploid plants were obtained.关键词
草莓/花药培养/单倍体育种Key words
strawberry/anther culture/haploid breeding分类
农业科技引用本文复制引用
赵永钦,吴新新,郑禾,路河,郭仰东..草莓‘甜查理’和‘章姬’花药培养及单倍体植株的获得[J].中国农业大学学报,2012,17(5):39-45,7.基金项目
国家重大基础研究973课题 （）
中央高校基本科研业务费专项 （）
