中国农业大学学报2012，Vol.17Issue(5)：46-53,8.
唐菖蒲丙二烯氧化物环化酶基因GhAOC的克隆与表达分析
Cloning and expression analysis of allene oxide cyclase gene GhAOC from Gladiolus hybridus
摘要
Abstract
To clone the full-length cDNA of GhAOC and analyze the expression patterns of GhAOC and the effect on the biosynthesis of jasmonic acid.The full length cDNA of GhAOC was cloned in Gladiolus hybridus’Rose Supreme’corms by RT-PCR and RACE.The technology of gene gun bombardment was used to analyze the sub-cellular localization of GhAOC.The real time RT-PCR was used to analyze the expression pattern of GhAOC.A full-length cDNA named GhAOC encoding a key enzyme of the biosynthesis of jasmonic acid was cloned in Gladiolus.The open reading frame encompassed 735 bp encoding a polypeptide of 244 amino acids with calculated protein molecular mass of 26.52 ku.The sub-cellular localization analysis indicated that GhAOC was a chloroplast protein.Real time RT-PCR analysis showed that GhAOC gene was expressed in leaf,flower,root,stolon,corm and cormel,and the relatively high expression level of GhAOC was observed in corm and cormel.Meanwhile,the expression level and the endogenous MJ content in corms steadily increased under MJ treatment with a raising concentration gradients from 0.1 mmol/L to 0.5 mmol/L.Based on the results of our pilot study,the expression of GhAOC promoted the the biosynthesis of jasmonic acid in Gladiolus hybridus.关键词
唐菖蒲/AOC基因/JA/克隆/表达Key words
Gladiolus hybridus/allene oxide cyclase/jasmonic acid/cloning/expression分类
农业科技引用本文复制引用
连青龙,李晓昕,钟雄辉,尹义蕾,义鸣放..唐菖蒲丙二烯氧化物环化酶基因GhAOC的克隆与表达分析[J].中国农业大学学报,2012,17(5):46-53,8.基金项目
公益性行业（农业）科研专项 （）
