中国农业科技导报2012，Vol.14Issue(3)：49-55,7.DOI:10.3969/j.issn.1008-0864.2012.03.08
一株对鳞翅目蔬菜害虫具有广谱高毒力的新Bt菌株
A Novel Bacillus thuringiensis Strain with Broad Spectrum and High Toxicity Against Lepidoptera Vegetable Pests
摘要
Abstract
Bacillus thuringiensis (Bt) strain MB-15,isolated from the soil in Hebei Province,was high toxic against Lepidoptera vegetable pests. Compared with Bt HD-1 ,the toxicities of Bt MB-15 against Helicoverpa armigera,Spodoptera exigua,Plutella xylostella,Spodaptera litura and Pieris rapae all were higher than that of Bt HD-1 by bioassay in lab. The supernatant of Bt MB-15 fermentation could remarkably enhance the insecticidal activity of Bt MB-15 crystal protein. The bipyramidal crystals were observed by microscopic observation in Bt MB-15 strain. SDS-PAGE analysis revealed that Bt MB-15 produced crystal proteins of 130. 0 kDa and 65. 0 kDa. Bt MB-15 contained cry1Ac,cry2Aa,cryll and vip3Aa gene by PCR-RFLP analysis. The results showed that Bt MB-15 could have the potential and commercial development value against Lepidoptera vegetable pests.关键词
苏云金芽胞杆菌/蔬菜害虫/鳞翅目/杀虫活性/基因型Key words
Bacillus thuringiensis/vegetable pests/Lepidoptera/insecticidal activity/genotype分类
农业科技引用本文复制引用
葛东华,苏俊平,宋萍,南宫自艳,王勤英..一株对鳞翅目蔬菜害虫具有广谱高毒力的新Bt菌株[J].中国农业科技导报,2012,14(3):49-55,7.基金项目
公益性农业行业项目(200903042-5)资助. （200903042-5）
