中国农业科技导报2012，Vol.14Issue(3)：138-144,7.DOI:10.3969/j.issn.1008-0864.2012.03.22
青藏高原云杉针叶水提物抑菌作用研究
Studies on Bacteriostasis of Needle Aqueous Extract of Picea asperata in Qinghai-Tibetan Plateau
摘要
Abstract
The antibacterial role of needle aqueous extract of Picea asperata (NAEPA) from Zoige in Qinghai-Tibetan Plateau was evaluated on 6 pathogenic bacteria,and its active compounds were investigated by gas chromatograph-mass spectrometry (GC-MS). In vitro antibacterial assay showed that NAEPA inhibited effectively both against Gram-positive and Gram-negative bacteria including Staphylococus aureus,Bacillus cereus,Bacillus subtilis,Escherichia coli,Klebsiella pneumoniae and Hafnia alvei,with minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations ( MBC) being 3.8 ~15 and 7.5 ~ 30 mg/mL,respectively. The antibacterial compounds were stable even after being heated to 121℃1 for 15 min. Antibacterial activity was the highest in acidic environment,but decreased when pH increased. The chemical composition of NAEPA assay revealed that organic acids were the main components. Benzoic acid,m-hydroxybenzoic acid,pyrocatechol,squalene and myrtenol were the main antibacterial compounds. In addition,NAEPA had antioxidant,lipid-lowering and antithrombotic compounds. Those results suggested that NAEPA might be a new potential source as natural antimicrobial agent applied in food and feed industries.关键词
青藏高原/云杉/抑菌作用/有机酸Key words
Qinghai-Tibetan Plateau/Picea asperata/antibacterial activity/organic acid分类
农业科技引用本文复制引用
李珍,林国秀,罗盛莲,游霞,丁聪聪,冯甦,张杰..青藏高原云杉针叶水提物抑菌作用研究[J].中国农业科技导报,2012,14(3):138-144,7.基金项目
“十一五”国家科技支撑计划项目(2009BAI84B01)资助. （2009BAI84B01）
