中国农业科技导报2012，Vol.14Issue(4)：135-141,7.DOI:10.3969/j.issn.1008-0864.2012.04.20
根癌农杆菌介导的籼稻蜀恢527遗传转化方法的探究
Studies on Methods of Agrobacterium-mediated Transformation of Indica Rice ( Shuhui 527 )
摘要
Abstract
The callus transformation rate of Indica rice in tissue culture is relatively poor. Especially,the establishment of highly efficient regeneration system of superior varieties and the factors which affect its transformation remain unknown. This situation restricts at certain extent the progress of Indica rice genetic engineering. The seeds of restorer line Shuhui527 were used in this study to investigate the effects of quality and physiological state of theirs mature embryo callus under different temperatures and light conditions. Meanwhile,transformation efficiency influenced by differentiation medium supplemented with different hormones and the ratio between hormones were further studied. The results showed that under light condition at 32℃ ,the mature embryo callus growth is the best,under N6D medium callus quality is the best after twice subcultures. The NBS medium adding 2. 0 mg/L KT +0. 02 mg/L NAA + 3 mg/L 6-BA + 50 mg/L VC + 0. 5 mg/L IAA was found most suitable for Shuhui 527 genetic transformation,and the differentiation rate reached 36. 42%. Using our new system,rice transcription factor OsbZIP39 was introduced into Indica rice genome by Agrobacterium-mediaied transformation. Resistant plants were obtained preliminarily by PCR analysis.关键词
根癌农杆菌/籼稻/愈伤组织/遗传转化Key words
Agrobacterium tumefaciens/Indica/callus/genetic transformation分类
农业科技引用本文复制引用
刘亮亮,唐维,喻旭,刘永胜..根癌农杆菌介导的籼稻蜀恢527遗传转化方法的探究[J].中国农业科技导报,2012,14(4):135-141,7.基金项目
国家杰出青年科学基金项目(30825030) （30825030）
国家转基因生物新品种培育重大专项(2009ZX08001-011B,2009ZX08009-072B)资助. （2009ZX08001-011B,2009ZX08009-072B）
