中国农业科技导报2012，Vol.14Issue(5)：33-41,9.DOI:10.3969/j.issn.1008-0864.2012.05.06
花生共生受体类蛋白激酶基因的克隆及生物信息学分析
Cloning and Bioinformatic Analysis of symrk Gene Cloned from Arachis hypogaea L
摘要
Abstract
Symbiosis receptor-like protein kinase (SYMRK) plays a key regulatory role in the symbiosis pathway of plant and microorganism. The full length and 560 bp upstream sequence of symrk gene were cloned from Arachis hypogaea Baisha1016 by genome-walking,respectively. Characters of the Ah-symrk and encoded amino acid residue sequence,including the general physical and chemical properties,transmembrane helices domains,localization sites in cells and senior structure,were predicted and analyzed by the methods of bioinformatics. The result showed that Ah-symrk contained 15 exons and 14 introns,and the cDNA full-length of Ah-symrk was 3 042 bp containing a complete ORF(2 781 bp) ,which encoded 926 amino acid. The protein kinase was a hydrophobie-acidic protein,which located in cell membrane. Three copies of root-motif-tapoxl related to root-specific expression were contained in upstream regulatory sequence. The result of RT-PCR showed that Ah-symrk was root-specific gene.关键词
基因组步移/共生受体类蛋白激酶/生物信息学/RT-PCRKey words
genome-walking/SYMRK/bioinformatics/RT-PCR分类
农业科技引用本文复制引用
段小红,耿丽丽,束长龙,朱延明,张杰..花生共生受体类蛋白激酶基因的克隆及生物信息学分析[J].中国农业科技导报,2012,14(5):33-41,9.基金项目
国家973计划项目(2009CB118902) （2009CB118902）
国家863计划项目(2011AA10A203)资助. （2011AA10A203）
