中国农业科学2012，Vol.45Issue(15)：3108-3114,7.
根皮苷对平邑甜茶根系TGA循环酶的影响
Effects of Phloridzin on the Tricarboxylic Acid Cycle Enzymes of Roots of Malus hupehensis Rehd.
摘要
Abstract
[Objective] A pot experiment was conducted to study the effects of phloridzin on the tricarboxylic acid cycle (TCA) of roots of Malus hupehensis Rehd. Which is used widely as apple common stocks. The mechanisms were discussed so as to provide a basis for further study on the cause of apple continuous cropping diseases. [Method] Phloridzin of 4 mmol·L-1 and KMnO4 of 1 mmol·L-1 were used in the pretreatment. Malus hupehensis Rehd. Were planted in pots and treated with phloridzin of 4 mmol·L-1 (T1) and phloridzin of 4 mmol·L-1 added with KMnO4 of 1 mmol·L-1 (T2). The content of phloridzin in the soil and respiratory rate of roots were determined. Activities of enzymes related to TCA including citrate synthase (CS), aconitase, isocitrate dehydrogenase (ICDH), α-ketoglutarate dehydrogenase (α-KGDH), succinate thiokinase (SCS), succinate dehydrogenase (SDH), malic dehydrogenase (MDH), fumarase (FUM) and pyruvate dehydrogenase (PDH) were also determined. [Result] Treatments with T1 inhibited the respiratory rate of roots of Malus hupehensis Rehd., decreased obviously enzyme activities for the most part including CS, aconitase, ICDH, SCS, SDH, FUM, MDH only except for a-KGDH and PDH that their enzymes activities were improved. However, treatments with T2 had a critical difference from T1, it significantly enhanced the activities of CS, aconitase and MDH even over the control level, increased obviously the activity of SCS close to the control level, and to some degree improved the activities of ICDH, SDH and FUM. Besides, the activities of α-KGDH and PDH of T2 treatments were assayed to be a lower level than T1 but higher than the control level. Furthermore, the content of phloridzin in soil of T2 was less than T1. [Conclusion] The results showed that phloridzin at the concentration of 4 mmol·L-1 could inhibit the respiratory rate and the enzyme activities (7 of 9) related to TCA of roots of Malus hupehensis Rehd. With the treatments time following. The treatment of 1 mmol·L-1 KMnO4 could ease the above-mentioned adverse impact.关键词
平邑甜茶/酚酸/根皮苷/TCA循环/KMnO4/连作Key words
Malus hupehensis Rehd./ phenolic acids/ phloridzin/ tricarboxylic acid cycle/ KMnO4/ continuous cropping引用本文复制引用
王青青,胡艳丽,周慧,展星,毛志泉,朱树华..根皮苷对平邑甜茶根系TGA循环酶的影响[J].中国农业科学,2012,45(15):3108-3114,7.基金项目
国家现代农业产业技术体系建设专项资金(CARS-28)、山东省农业重大应用技术创新课题联合资助 （CARS-28）
