中国农业科学2012，Vol.45Issue(16)：3288-3296,9.DOI:10.3864/j.issn.0578-1752.2012.16.008
可感染茶尺蠖的重组家蚕核型多角体病毒的构建
Construction of Recombinant BmNPV Infecting Ecotropis oblique
摘要
Abstract
[Objective] The objective of this study is to construct recombinant BmNPV infecting both silkworm and Ecotropis oblique, and to solve the reproduction difficulty of EoNPV because of the breeding complexity. [Method] Recombinant BmNPV containing the polh expression box of BmNPV and the hel expression box of EoNPV were constructed by Bac-to-Bac system. [Result] rBmNPV amplified from silkworm or BmN cell could infect E. oblique, which indicated the host domain of rBmNPV had been broaden to E. oblique. [Conclusion] rBmNPV which could infect E. oblique was successfully constructed, thus laied a foundation of producing recombinant virus pesticide using silkworm as host.关键词
EoNPV/BmNPV/重组病毒/宿主域Key words
EoNPV/ BmNPV/ recombinant virus/ host domain引用本文复制引用
郭睿,刘卫星,潘中华,薛仁宇,曹广力,朱越雄,贡成良..可感染茶尺蠖的重组家蚕核型多角体病毒的构建[J].中国农业科学,2012,45(16):3288-3296,9.基金项目
国家自然科学基金项目(31072085)、江苏省自然科学基金项目(BK2009117)、江苏省"六大人才高峰"项目(2009-D-Ag18)、苏州市自然科学基金项目(YJG0907)、苏州大学重大应用研究培育项目(Q3134991) （31072085）
