中国农业科学2012，Vol.45Issue(16)：3430-3436,7.DOI:10.3864/j.issn.0578-1752.2012.16.024
蒺藜苜蓿低温胁迫响应基因MtbHLH-1的克隆及功能分析
Isolation and Functional Analysis of the Low-Temperature-Responsive Gene MtbHLH-1 in Medicago truncatula
摘要
Abstract
[Objective] This study is aimed to characterize the low-temperature-responsive gene MtbHLH-1 in M. truncatula by clone and analyse its sequence, and study the function of MtbHLH-1 in low temperature response. [Method] The full-length cDNA sequence of MtbHLH-1 was isolated by RT-PCR. The obtained cDNA sequence and the deduced amino acid sequence were analysed. Semi-quantitative RT-PCR was used to assess the expression of MtbHLH-1 in response to low temperature (4℃) stress. [ Result ]The sequencing result showed that the cloned cDNA (designated as MtbHLH-1, GenBank Accession No. JN800447) was 778 bp, contains an open reading frame (ORF) of 741 bp, and encoding a protein with 246 amino acids. BLASTp search revealed 45% to 73% similarities between the MtbHLH-1 protein and those reported bHLHs from other species, such as Glycine max, Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum and Zea mays. The qRT-PCR result showed that MtbHLH-1 was expressed upregulatedly in response to low temperature stress. [Conclusion] The cold stress response transcription factor MtbHLH-1 was cloned from M. truncatula and the semi-quantitative analysis of the gene MtbHLH-1 under low temperature demonstrate that the MtbHLH-1 gene probably improved low temperature stress tolerant capacities.关键词
蒺藜苜蓿/MtbHLH-1/bHLH转录因子/功能分析Key words
Medicago truncatula/ MtbHLH-1/ bHLH transcription factors/ functional analysis引用本文复制引用
李宇伟,连瑞丽,王新民..蒺藜苜蓿低温胁迫响应基因MtbHLH-1的克隆及功能分析[J].中国农业科学,2012,45(16):3430-3436,7.基金项目
河南省杰出青年科学基金项目(074100510018)、河南省重大科技攻关项目(072201120001) （074100510018）
