中国全科医学2012，Vol.15Issue(23)：2664-2666,3.DOI:10.3969/j.issn.1007-9572.2012.08.057
贵州省艾滋病患者耐药基因突变研究
Drug Resistance Gene Mutation in AIDS Patients in Guizhou Province
摘要
Abstract
Objectives To study drug resistance gene mutation in AIDS patients, to provide scientific base for prevention of the spreading and prevalence of AIDS drug resistance. Methods Bio - Maria NueliSens EasyQ fluorescence real -time quantitative PCR system was used to determine viral load in AIDS patients treated over 1 year. An analysis of amplification, sequencing and gene mutation was performed, by ViroSeqTMHIV - 1 Genotyping System V2. 0 system made by Abbott, in patients with viral load > 1 000 U/ml. Results In 1 088 AIDS patients, Viral load was > 1 000 U/m in 45, 30 gained valid gene sequence of POL with varying degrees of gene mutations, only 7 had drug resistance, the total drug resistance rate being 0. 64% ; 2 cases had cross resistance. More than one kind of drug - resistance related gene mutable point were noted in the 7 patients with drug resistance, with a mutable rate of 23. 3% ( 7/30 ). AIDS patients had drug resistance gene mutation to non -nucleoside reverse transcriptase inhibitor ( non - NRTI ), and its mutable points were K103N ( 4 cases ), G190A ( 2 cases ), V179D ( 1 case), Y181C ( 1 case), K101E ( 1 case), M230L ( 1 case), clinically highly resistant to DLV, EFV, NVP. Two patients had drug resistance gene mutation to non - NRTI, M184V being mutable point and clinically highly resistant to telbi-vudine and emtricitabine. Drug resistance gene mutation to proteinase inhibitors was not noted. Conclusions Situation of drug resistance is at a low level with relatively simple drug resistance form. NRTI and non - NRTI is still effective in antiviral treatment of AIDS in Guizhou Province. Timely monitoring and grasp of drug resistance gene mutation of AIDS patients are important to guidance to clinical medication.关键词
获得性免疫缺陷综合征/抗病毒药/DNA突变分析Key words
Acquired immunodeficiency syndrome/ Antiviral agents/ DNA mutational analysis分类
医药卫生引用本文复制引用
孙伟,李志坚,孙显光,卜朴..贵州省艾滋病患者耐药基因突变研究[J].中国全科医学,2012,15(23):2664-2666,3.基金项目
贵州省"十一五"重点攻关社会发展资金项目(黔科合字NY[2006]-3051) （黔科合字NY[2006]-3051）
