中国全科医学2012，Vol.15Issue(27)：3146-3148,3.DOI:10.3969/j.issn.1007-9572.2012.09.091
氨基胍对糖基化终产物干预后人肾系膜细胞fractalkine表达的影响
Effect of Aminoguanidine on Fractalkine Expression of AGE-Treated Human Renal Mesangial Cells
魏琼 1孙子林 1金晖 1王少华 1张晓黎 1金虹 1魏芹1
作者信息
- 1. 210009 江苏省南京市,东南大学附属中大医院内分泌科
- 折叠
摘要
Abstract
Objective To investigate the effect of aminoguanidine (AG) on fractalkine expression of advanced glyeosylation end products (AGEs) treated human renal mesangial cells (HRMC) . Methods HRMC were incubated with DMEM, bovine serum albumin (BSA) and AGE-BSA respectively, as well as the combination of different concentrations of AG and AGE-BSA for 24 hours. The mRNA and protein expressions of fractalkine in HRMC were tested by RT-PCR and Westernblot respectively. Results The expression levels of fractalkine mRNA and protein in HRMC treated with AGE-BSA were significantly higher than those in HRMC treated with DMEM and BSA (P <0. 05).The down-regulation of mRNA and protein in HRMC by AG was concentration dependent (P<0. 05) . Conclusion AG might play its renal protection role via the attenuation of the up-regulation of fractalkine by AGEs.关键词
氨基胍/糖尿病肾病/糖基化终产物/FractalkineKey words
Aminoguanidine/ Diabetic nephropathy/ Advanced glycosylation end products/ Fractalkine分类
医药卫生引用本文复制引用
魏琼,孙子林,金晖,王少华,张晓黎,金虹,魏芹..氨基胍对糖基化终产物干预后人肾系膜细胞fractalkine表达的影响[J].中国全科医学,2012,15(27):3146-3148,3.
