中国水产科学2012，Vol.19Issue(5)：741-755,15.DOI:10.3724/SP.J.1118.2012.00741
鲤多拷贝基因Apo-14kDa和ApoE的系统学分析和其在胚胎发育中的功能探索
Phylogenetic analysis and expression during embryonic development of multiple copies of Apo-14 kDa and ApoE genes in common carp,Cyprinus carpio
摘要
Abstract
Apolipoproteins, the protein moieties of lipoproteins, participate in physical activities in different forms. A 14 kDa apolipoprotein (Apo-14 kDa) and apolipoprotein E (ApoE) are thought to be associated with embryonic development in animals. We analyzed the gene structure and phylogenetic evolution of these two types of apolipoprotein in the common carp {Cyprinus carpio). In addition, we documented the pattern of expression using RT-PCR to provide insight into their function during the embryonic development of carp. We obtained two types of Apo-14 kDa and four types of ApoE, including Apo-14kDa-a and Apo-14kDa-b (identity=79%), ApoE-al and ApoE-a2 (identity=91%), and ApoE-bl and ApoE-b2 (identity=90%). Phylogenetic analysis revealed that Apo-14 kDa-a has a close relationship with that of most other teleosts and that Apo-14 kDa-b has the closest relationship with that of grass carp. These relationships likely reflect the early differentiation of the family Cyprinidae, although ApoE is characterized by four homologous genes. The expression of carp Apo-14 kDa-a was highest in the liver and head-kidney, was very low in the intestine, and undetectable in the remaining tissues. Conversely, we were only able to detect expression of Apo-14 kDa-b in the liver. We observed tissue specific differences in the expression of Apo-14 kDa-a and Apo-14 kDa-b. ApoE-al and ApoE-a2 were only detected in the intestine whereas ApoE-bl and ApoE-b2 were present in the brain, skin, gill, liver, spermary, intestine, heart, muscle, body-kidney, and head-kidney. ApoE-bl was also present in the spleen and ApoE-b2 in the ovary. Our observations suggest there is considerable variation in the expression and function of ApoE. Furthermore, qRT-PCR analysis suggests that the expression of these six genes varies throughout embryonic development, both within and among genes. This is consistent with the molecular phylogeny of these genes. qRT-PCR analysis suggests that Apo-14 kDa and ApoE play an important role throughout different stages of embryonic development in common carp.关键词
鲤/载脂蛋白/Apo-14kDa/ApoE/分子系统学/胚胎发育/基因功能Key words
Cyprinus carpio/ apolipoprotein/ Apo-14kDa/ ApoE/ paralogs/ molecular phylogeny/ embryonic development/ gene function分类
农业科技引用本文复制引用
崔润滋,万玉美,孙金生,汪金兔,徐鹏,孙效文..鲤多拷贝基因Apo-14kDa和ApoE的系统学分析和其在胚胎发育中的功能探索[J].中国水产科学,2012,19(5):741-755,15.基金项目
国家863计划项目(2009AA10Z105,2011AA100401) （2009AA10Z105,2011AA100401）
农业部公益性行业科研专项项目(200903045) （200903045）
中央级公益性科研院所基本科研业务费专项资金项目(2009B002). （2009B002）
