中国水产科学2012,Vol.19Issue(5):806-812,7.DOI:10.3724/SP.J.1118.2012.00806
中华绒螯蟹蜕皮周期及蜕皮过程中肝胰腺消化酶活性的变化
Molt stages and changes in digestive enzyme activity in hepatopancreas during molt cycle of Chinese mitten crab, Eriocheir sinensis
摘要
Abstract
The molt cycle of the Chinese mitten crab Eriocheir sinensis was staged by the method of Darch. We then measured the three digestive enzymes in hepatopancreas throughout the molt cycle. Based on the setogenesis and the morphological changes to the epidermis of the third maxilliped, we identified four molting periods (post-molt, intermolt, premolt and ecdysis). In addition, the premolt period was further divided into three substages (D0, D1, and D3-4). Both amylase and tryptase activities increased in the hepatopancreas during the A-B stages, peaking in the early premolt D0 stage (P<0.05), and then gradually decreasing in the later premolt D3-4 stage (.P<0.05). There were no obvious changes in peptase activity during the majority of the molt cycle, though levels were significantly lower (P<0.05) in the later premolt D3-4 stage. In summary, we identified five periods (C, D0, D1, D3-4 A-B)in the molt cycle of E. sinensis during which the activities of amylase, trypsase, and peptase changed periodically.关键词
中华绒螯蟹/蜕皮周期/形态学/肝胰腺/消化酶Key words
Eriocheir sinensis/ molt cycle/ morphology/ hepatopancreas/ digestive enzyme分类
农业科技引用本文复制引用
康现江,田志环,吴江立,穆淑梅..中华绒螯蟹蜕皮周期及蜕皮过程中肝胰腺消化酶活性的变化[J].中国水产科学,2012,19(5):806-812,7.基金项目
河北省自然科学基金项目(C2011201028). (C2011201028)