中国水产科学2012，Vol.19Issue(5)：821-832,12.DOI:10.3724/SP.J.1118.2012.00821
云纹石斑鱼(♀)×七带石斑鱼(♂)杂交子一代胚胎发育及仔稚幼鱼形态学观察
Embryonic and morphological development in larva, juvenile, and young stages of F1 by Epinephelus moara (♀)×E.septemfasciatus (♂)
摘要
Abstract
Hybridization is the most widely used and effective method of artificial breeding in aquaculture. To develop grouper species that were fast growing and tolerant of low temperatures, we conducted hybridization between Epinephelus moara (♀) and E. septemfasciatus (♂) by artificial methods. We documented embryonic development and the growth and morphological characteristics of larva, juveniles, and young fish in the hybrid F1. The embryonic development of the hybrid Fl was divided into cleavage stage, blastula stage, gastrula stage, neu-rula stage, and organogenesis stage. In sea-water (21-22.5℃, salinity 29, pH 8-8.1), 35 h 20 min was required for hybrid Fl embryonic development. The 2-cell stage occurred 1 h post fertilization (pf); the 16-cell stage at 2 h 25 min pf; the multi-cell stage at 3 h 50 min pf; the high blastula stage at 5 h 10 min pf; the middle gastrula stage at 12 h 40 min pf; the embryo body stage at 16 h pf; the closure of blastopore stage at 17 h 50 min pf; the muscle burl stage at 20 h 50 min pf; the brain vesicle stage at 24 h 50 min pf; the crystal stage at 31 h 05 min pf; the heart-beating stage at 32 h 40 min pf; the hatching stage at 35 h 10 min pf; and the newly hatched larvae stage at 35 h 20 min. There was no obvious difference in embryonic development between crossbreed Fl by E. moaraxE.septemfasciatus and E. moara, except the time of the heart-beating stage. Based on the features of the yolk sac, second dorsal fin spine, pelvic fin spine, scales, and body color, the post-embryonic development of the hybrid Fl was divided into larval, juvenile, and young fish stages. The larvae relied on their yolk for nourishment up to 4 d after hatching, at which time the mouth opened. Metamorphosis was completed ~42±2 d after hatching at which time the fish were classified as juveniles. The juvenile stage lasted for ~58±4 d, after which the juveniles became young fish. Our results suggest that the hybrid Fl grows more rapidly than either parent species.关键词
云纹石斑鱼/七带石斑鱼/杂交子一代/胚胎发育/形态观察Key words
Epinephelus moara/ E. septemfasciatus/ crossbreed Fl/ embryonic development/ morphological observation分类
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李炎璐,王清印,陈超,翟介明,吴雷明,马文辉,宋振鑫,孙芳芳..云纹石斑鱼(♀)×七带石斑鱼(♂)杂交子一代胚胎发育及仔稚幼鱼形态学观察[J].中国水产科学,2012,19(5):821-832,12.基金项目
科技部国际合作项目(2012DEA30360). （2012DEA30360）
