中国水产科学2012，Vol.19Issue(5)：872-880,9.DOI:10.3724/SP.J.1118.2012.00872
浒苔腐烂过程中水体细菌群落结构变化的PCR-DGGE分析
Changes in population structure of bacteria during decomposition of Enteromorpha prolifera
摘要
Abstract
We used ecological simulation to study changes in the population structure of bacteria in water during the decomposition of Enteromorpha prolifera. We placed 3.0 kg of dead E. prolifera into a 500 L glass tank and measured chemical oxygen demand (COD) and dissolved oxygen (DO). In addition, we counted the number of aerobic heterotrophic bacteria and analyzed the structure of the bacteria population at different stages using PCR-DGGE. We observed an initial increase in DO, COD, and the abundance of aerobic heterotrophic bacteria as the degree of decomposition of E. prolifera increased, followed by a decrease in COD and aerobic heterotrophic bacteria abundance. The bacterial population in the water consisted primarily of Flavobacteria ?9.39%) and Pro-teobacteria (60.61%). We observed a significant change in the species structure during the decomposition process. During the initial stage of decomposition, Flavobacteria were dominant, but were replaced by a-, y-, and e-Proteobacteria during the latter stages of decomposition. Our results provide insight into predicting the influence of E. prolifera breakouts in marine environment.关键词
浒苔/细菌群落/生物多样性/PCR-DGGEKey words
Enteromorpha prolifera/ bacteria population/ biodiversity/ PCR-DGGE分类
农业科技引用本文复制引用
张艳,李秋芬,孙雪梅,陈聚法,赵俊..浒苔腐烂过程中水体细菌群落结构变化的PCR-DGGE分析[J].中国水产科学,2012,19(5):872-880,9.基金项目
海洋公益性行业科研专项经费项目(200805069) （200805069）
青岛市科技计划项目(08-1-7-6-hy) （08-1-7-6-hy）
中央级公益性科研院所基本科研业务费项目(2010-chb-01). （2010-chb-01）
