中国水产科学2012，Vol.19Issue(5)：900-905,6.DOI:10.3724/SP.J.1118.2012.00900
氢化物发生-原子荧光光谱法测定水产品中有机硒和无机硒
Determination of organic and inorganic selenium in aquatic products by hydride generation- atomic florescence spectrometry
摘要
Abstract
We measured the total selenium content in aquatic product samples after oxidation of organic selenium into inorganic selenium using a nitric acid, perhydrol mixed system. Simultaneously, we measured inorganic selenium content by HG-AFS following extraction of the sample in a HCL(50%) solution and digestion of the water phase in HNO3 and H2O2. Using these data we calculated the organic selenium content of the samples. Under optimal conditions, the detection limit of selenium by this method was 7.1 ng/mL and the linear range was between 0-20 ug/L. The recovery rate of total selenium was 93.88%-107.65% and the RSD was 0.82%-4.97%. Similarly, the recovery rate of organic selenium was 82.88%-90.10% and the RSD was 2.33%-7.73%. In summary, this method has a wide linear dynamic range and good precision and accuracy, so is suitable for the determination of organic and inorganic selenium in aquatic products.关键词
原子荧光光谱法/水产品/无机硒/有机硒Key words
HG-AFS/ aquatic products/ inorganic selenium/ organic selenium分类
农业科技引用本文复制引用
张美琴,季华曼,杨洪生,孟勇,王静,吴光红..氢化物发生-原子荧光光谱法测定水产品中有机硒和无机硒[J].中国水产科学,2012,19(5):900-905,6.基金项目
江苏省科技基础设施建设计划项目(BM2008158). （BM2008158）
