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Progesterone promotes neuronal differentiation of human umbilical cord mesenchymal stem cells in culture conditions that mimic the brain microenvironment

Xianying Wang Honghai Wu Gai Xue Yanning Hou

中国神经再生研究（英文版）2012，Vol.7Issue(25)：1925-1930,6.

中国神经再生研究（英文版）2012，Vol.7Issue(25)：1925-1930,6.DOI:10.3969/j.issn.1673-5374.2012.25.001

Progesterone promotes neuronal differentiation of human umbilical cord mesenchymal stem cells in culture conditions that mimic the brain microenvironment

Xianying Wang ¹Honghai Wu ²Gai Xue ³Yanning Hou³

作者信息

1. Hebei Medical University, Shijiazhuang 050017, Hebei Province, China
2. The Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei Province, China
3. Bethune International Peace Hospital of Chinese PLA, Shijiazhuang 050082, Hebei Province, China
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摘要

Abstract

In this study, human umbilical cord mesenchymal stem cells from full-term neonates born by vaginal delivery were cultured in medium containing 150 mg/mL of brain tissue extracts from Sprague-Dawley rats (to mimic the brain microenvironment). Immunocytochemical analysis demonstrated that the cells differentiated into neuron-like cells. To evaluate the effects of progesterone as a neurosteroid on the neuronal differentiation of human umbilical cord mesenchymal stem cells, we cultured the cells in medium containing progesterone (0.1, 1, 10 μM) in addition to brain tissue extracts. Reverse transcription-PCR and flow cytometric analysis of neuron specific enolase-positive cells revealed that the percentages of these cells increased significantly following progesterone treatment, with the optimal progesterone concentration for neuron-like differentiation being 1 μM. These results suggest that progesterone can enhance the neuronal differentiation of human umbilical cord mesenchymal stem cells in culture medium containing brain tissue extracts to mimic the brain microenvironment.

关键词

progesterone/ mesenchymal stem cells/ neuron/ differentiation/ brain tissue extracts/ neural regeneration

Key words

progesterone/ mesenchymal stem cells/ neuron/ differentiation/ brain tissue extracts/ neural regeneration

引用本文复制引用

Xianying Wang,Honghai Wu,Gai Xue,Yanning Hou..Progesterone promotes neuronal differentiation of human umbilical cord mesenchymal stem cells in culture conditions that mimic the brain microenvironment[J].中国神经再生研究（英文版）,2012,7(25):1925-1930,6.

基金项目

This study was supported by the Military Medical Research Program during the 12th Five-Year Plan Period,No.BWS11J002. （）

中国神经再生研究（英文版）

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ISSN：1673-5374

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