首页|期刊导航|中国神经再生研究（英文版）|Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application
中国神经再生研究（英文版）Issue(30)：2347-2353,7.DOI:10.3969/j.issn.1673-5374.2012.30.004
Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application
Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application
摘要
Abstract
In this study,we extracted and purified components in the Angong Niuhuang pill.Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology.The Angong Niuhuang sticker includes extracts from curcuma,berberine hydrochloride,baicalin,geniposide,borneol,and musk.Angong Niuhuang stickers at different point application doses (1.35,2.7,and 5.4 g/kg) were administered to Dazhui (DU14),Qihai (RN6) and Mingmen (DU4).Rats in the different dose point application and acupuncture groups were continuously administered for 7 days.Then a middle cerebral artery occlusion model was prepared for simulating human cerebral ischemia.Twelve hours later,expressions of Bcl-2,Bax and p53 protein in hippocampal CA1 were detected with immunohistochemistry.The expression level of Bcl-2,an anti-apoptotic protein,significantly increased in the high-,medium-and low-dose point application groups and the acupuncture group,while the expression of the pro-apoptotic proteins,Bax and p53,significantly decreased compared with the middle cerebral artery occlusion rats; and the Bcl-2/Bax ratio was significantly increased.The difference was noticeable for the high-dose point application group,which showed statistical difference compared with the low-dose point application group and the acupuncture group.Our experimental findings indicate that point application with Angong Niuhuang stickers promotes the expression of Bci-2,and inhibits the expressions of Bax and p53 in the hippocampal CA1 area of rats after focal cerebral ischemia.Thus,point application of Angong Niuhang stickers protects brain tissues from cerebral ischemia.关键词
cerebral ischemia/ point application/ acupuncture/ Angong Niuhuang sticker/ Bcl-2/ p53/ Bax/apoptosis/ brain injury/ neural regenerationKey words
cerebral ischemia/ point application/ acupuncture/ Angong Niuhuang sticker/ Bcl-2/ p53/ Bax/apoptosis/ brain injury/ neural regeneration引用本文复制引用
Dongshu Zhang,Maodong Fu,Chenglong Song,Caiyun Wang,Xiaochun Lin,Yuanliang Liu..Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application[J].中国神经再生研究（英文版）,2012,(30):2347-2353,7.基金项目
This study was supported by the Research Project of Administration of TCM of Guangdong Province,No.20111270. （）
