中国生物防治学报2012,Vol.28Issue(4):514-520,7.
昆虫病原线虫对甘薯蚁象的致病力测定
Virulence of Entomopathogenic Nematodes against Sweetpotato Weevils
摘要
Abstract
Entomopathogenic nematodes (EPN) are effective biological control factors against cryptic pests. Virulence of 8 Steinernema spp. and 3 Heterorhabditids spp. against last instar larvae of sweetpotato weevils Cylas formicarius were evaluated and Steinernemafeltiae showed the highest virulence. Then the virulence of 11 S. feltiae strains originated from different geographical locations were tested, S. feltiae JY-17 was the best one against the last instar larvae of C. formicarius. Effect of different factors on the virulence of S. feltiae JY-17 to C. formicarius was determined. The results showed that the sensitivity of insect stages at 25 ℃ were in a sequence of last instar larvae 〉young larvae 〉pupa〉pre-pupa〉 adult; the virulence of S. feltiae JY-17 to C. formicarius were in an order of 25 ℃ 〉20 ℃ 〉 30 ℃ 〉 15 ℃ 〉 10 ℃ 〉 35 ℃. The LDs0 and LD90 of nematodes to the last instar larvae of C. formicarius were 5.532 IJs.larva-1 and 28.049 IJs.larva&-1 at 25 ℃ and in the density of 196.6 larva.m^-2 respectively. LTs0 of nematodes to the last instar larvae of C. formicarius was 44.306 h when the ratio of nematode to insect was 5 : 1. The present study provides some useful information on the biocontrol of sweetootato weevils with EPN.关键词
昆虫病原线虫/甘薯蚁象/致死率/侵入率Key words
entomopathogenic nematodes/Cylasformicarius/mortality/invasion rate分类
农业科技引用本文复制引用
于海滨,马娟,王容燕,耿亚玲,陈书龙..昆虫病原线虫对甘薯蚁象的致病力测定[J].中国生物防治学报,2012,28(4):514-520,7.基金项目
国家甘薯产业技术体系 ()