中国生物防治学报2012，Vol.28Issue(4)：545-551,7.
哈茨木霉对接种尖孢镰刀茵后黄瓜根系次生代谢物的影响
Effects of Trichoderma harzianum on Secondary Metabolites in Cucumber Roots Infected With Fusarium oxysporum
摘要
Abstract
Interaction between Trichoderma harzianum and cucumber Fusarium wilt pathogen was studied in vitro and in vivo. Three tested isolates of T. harzianum exhibited distinct inhibitory effects on Fusarium in dual tests. Isolate TN showed performed more inhibitively than the other tested isolates. Field inoculation of T. harzianum resulted in enhanced content of flavonoids, phenolics and lignin in cucumber plants. Meanwhile, the activities of CAD, PPO, G-POD, CA-POD and CGA-POD were increased by 100%, 54%, 278%, 143% and 52%, respectively. The expression level of resistance-related genes in plants inoculated with T. harzianum was higher than that of the control. The expression levels of all selected genes but CCOMT were higher on 6 dai (days after inoculation) than those on 2 dai. The expression levels of C4H, CAD, CCOMT, G6PDH, PAL andPR-1 in the T. harzianum colonized plants were 5.64, 5.31, 4.28, 15.33, 7.36 and 6.45 times higher than that in control, respectively. Our findings demonstrated the close relationship between secondary metabolism and the T. harzianum-mediated induced resistance towards Fusarium infection in cucumber roots.关键词
哈茨木霉/拮抗作用/黄瓜枯萎病/次生代谢Key words
Trichoderma harzianum/antagonism effect/cucumber Fusarium wilt disease/secondary metabolism分类
农业科技引用本文复制引用
刘爱荣,陈双臣,晋文娟,于贝贝,王凤华,贺超兴..哈茨木霉对接种尖孢镰刀茵后黄瓜根系次生代谢物的影响[J].中国生物防治学报,2012,28(4):545-551,7.基金项目
国家科技支撑计划 （）
国家自然科学基金 （）
河南省青年骨干教师计划 （）
河南省教育厅自然科学基金 （）
