中国输血杂志2012，Vol.25Issue(8)：741-744,4.
病毒灭活血浆对人CIK细胞功能影响的体外实验研究
In vitro study of the effect of virus inactivated plasma on the function of human CIK cells
摘要
Abstract
Objective To study whether the immune cells could be affected by the virus inactivated plasma,which u-sing methylene blue light chemical method,via CIK cells. Methods The peripheral mononuclear cells of 10 healthy blood donors were used to culture human CIK by using virus inactivated plasma and fresh frozen plasma. And the amplification of CIK cells was observed in the two groups. The CD3 + CD56 + expression on CIK cells was detected via flow cytometry( FCM).The variation of perforin and granzyme B content, which were induced by Resveratrol with a final concentration of 0. 8 μmol/L for 48 hours were also detected. LDH assay was used to measure the CIK cell killing activity of SCG7901 cells. Results The proliferation multiples of CIK cell, cultured by fresh frozen plasma and virus inactivated plasma at 5 ,10 and 15 days, were 17.62 ± 1.88,26. 31 ± 1.95 ,46.05 ±2. 86, and 18.10 ± 1. 73,25. 97 ± 1.55,45. 82 ± 1. 15 ,respectively. The expression of CD3 + CD56 + and the promotion of CIK cell increment showed no significant difference between the two groups ( P > 0. 05 ). The perforin, granzyme B and killing activity of CIK cells in the fresh frozen plasma group and virus inactivated plasma group were(37.17 ± 1. 95)% , (38. 79 ± 1. 91)% , (46. 05 ±2. 86)%, and (32. 04 ± 1. 92)% , (33. 50 ± 1. 17)%,(45. 82 ±1.15)% .respectively. There was also no significant difference between 2 groups (P >0.05). Conclusion Virus inactivated plasma had no effect on CIK cell proliferation, perforin, Granzyme B content and killing activity in vitro.关键词
病毒灭活血浆/新鲜冰冻血浆/CIK细胞/增殖/杀伤功能Key words
Virus inactivated plasma/ Fresh frozen plasma/ CIK cells/ Proliferation/ Killing function分类
医药卫生引用本文复制引用
姚仁南,陈玲,刘军权,周忠海,陈娜云,陈复兴..病毒灭活血浆对人CIK细胞功能影响的体外实验研究[J].中国输血杂志,2012,25(8):741-744,4.基金项目
本课题受南京军区医学科技创新重点课题(08Z009)资助 （08Z009）
