中国实验动物学报2012，Vol.20Issue(4)：9-15,7.DOI:10.3969/j.issn.1005-4847.2012.04.002
奖励性操作式条件反射任务在大鼠学习记忆研究中的应用
Evaluation of reward-relevant learning and memory behavior with operant conditioning task in rats
摘要
Abstract
Objective To apply reward directed operant conditioning task for learning and memory research in laboratory animals. Methods Sixteen adult male SHR rats and 8 Wistar rats were included in this study. Operant conditioning procedure was used to assess the learning ability of Wistar and SHR rats with magazine training, fixed ratio, signal discrimination and extinction procedure. Results Significant difference was found in magazine training. The SHR rats performed more NP vs. Wistar rats and SHR rats treated with donepezil ( P < 0. 05 ). There was no significant difference during FR1 schedule. While in higher response ratio FR2 and FR4 schedule, the SHR group showed significantly higher error responses ( vs. Wistar rat group, P < 0. 05 ) , lower rewards eared ( vs. Donepezil-treated SHR rat group, P < 0. 001 ) , and significantly lower correct accuracy rate ( vs. Wistar rats and Donepezil-treated SHR rats, P <0. 05 ). Significantly higher error responses were found during signal discrimination task (vs. Wistar rats and Donepezil-treated SHR rats, P <0. 05) , and lower correct accuracy rate ( vs. Wistar rats and Donepezil-treated SHR rats, P < 0. 05). No significant difference was found during extinction. Neurotransmitter test showed that the hippocampal content of glutamale [( 1.0639 ±0.07086) mg/g ] , acetylcholine [ ( 2. 7760 ± 0. 2609 ) μg/g ] and 5 -HT [ ( 1. 2200 ± 0. 1137 ) μg/g ] were significantly lower in the SHR group ( vs. Wistar rat group, P < 0. 05 ) . The content of acetylcholine [ ( 3. 9344 ± 0. 2747 ) μg/g] was higher in the Donepezil-treated SHR rats group ( vs. Wistar rats,P < 0. 05 ; vs. SHR rats, P < 0. 001 ). Conclusions Reward directed operant conditioning is a type of positive reinforced conditioning. This task can be used as a new behavior test method for learning and memory study.关键词
操作式条件反射/奖赏/Wistar大鼠/SHR大鼠/学习记忆Key words
Operant conditioning/ Reward/ Wistar rats/SHR rats/Learning/ Memory分类
生物科学引用本文复制引用
石哲,曲丽娜,刘新民,陈善广,陈玲玲,孙秀萍,宋广青,王琼,常琪,李海清,李莹辉..奖励性操作式条件反射任务在大鼠学习记忆研究中的应用[J].中国实验动物学报,2012,20(4):9-15,7.基金项目
国家自然科学基金面上项目(30973888) （30973888）
国家科技重大专项新药创制专项:(2009ZX09502-014) （2009ZX09502-014）
国家航天医学与基础应用重点实验室开放课题(SMFA12B05). （SMFA12B05）
