中国实验动物学报2012，Vol.20Issue(4)：31-34,4.DOI:10.3969/j.issn.1005-4847.2012.04.006
副溶血弧菌毒力评价小鼠模型的建立与应用
Establishment and application of a mouse infection model for evaluating the virulence of Vibrio parahaemolyticus
摘要
Abstract
Objective To establish a mouse infection model for evaluating the virulence of Vibrio parahaemolyticus. Methods 4-5-week old female Balb/c mice were monitored for symptoms and lethality after being infected intraperito-neally with Vibrio parahaemolyticus bacterial suspension. Results The survival rate of mice after i. p. infection with a highly virulent RIMD2210633 strain (107CFU) cultivated in a high salt medium (2% NaCI) was 20-30% , while that with environmental non-virulent S251 strain was 100%. Conclusions We have successfully established a mouse infection model using the RIMD2210633 strain. This mouse model is suitable for evaluating the Vibrio parahaemolyticus virulence, and is used to compare the virulence of highly virulent and environmental non-virulent strains cultured in medium at different salt concentrations.关键词
副溶血弧菌/小鼠/致死性Key words
Vibrio parahaemolyticus/ Infection/ Lethality/ Mouse分类
生物科学引用本文复制引用
郭李平,徐镔蕊,杜德燕,姜汉雯,邱业峰,周冬生..副溶血弧菌毒力评价小鼠模型的建立与应用[J].中国实验动物学报,2012,20(4):31-34,4.基金项目
国家自然科学基金(30930001、30900823)和973项目(2009CB522604). （30930001、30900823）
