中国实验动物学报2012，Vol.20Issue(4)：80-86,7.DOI:10.3969/j.issn.1005-4847.2011.04.017
乳酸调控大鼠肠黏膜微血管内皮细胞的NF-κB信号通路
Lactic acid inhibits the NF-κB signaling pathways in intestinal mucosal microvascular endothelial cells of rats
摘要
Abstract
Objective The aim of this study was to explore the effects over time of lactic acid (LA) on IκBα phosphorylation and expressions of nuclear factor-kappa B p65 protein, tumor necrosis factor a (TNF-a) and interleukin-6 (IL-6) mRNA in the NF-κB signaling pathway in rat intestinal mucosal microvascular endothelial cells stimulated by li-popolysaccharide (LPS) , and then to assess the optimal action time of LA and its regulating site in the NF-kB signaling pathways. Methods IκBα, phosphorylated IκBα (p-IκBα) and NF-kB p6S proteins were monitored by Western blot analysis, and the TNF-a and IL-6 mRNA was analyzed using real-time PCR. Results LA treatment reduced TNF-a and IL-6 mRNA expression in LPS-stimulated RIMMVECs, with the greatest effect after 24 h and 3 h. The highest inhibitory effect of LA on IκBα phosphorylation and NF-kB transcriptional activity was during 4 to 8 h. The site of action of LA was IκBα phosphorylation in the signaling pathways. Conclusions The results of this study suggest that LA inhibits downstream inflammatory cytokine expression through inhibiting IκBα phosphorylation and blocking activation of NF-kB, thus playing a key anti-inflammatory role in the intestinal mucosal microvascular endothelial cells in rats.关键词
乳酸/内毒素/大鼠肠黏膜微血管内皮细胞/NF-κBKey words
Lactic acid/ lipopolysaccharide/ Intestinal mucosal endothelial cells/ Signaling pathway, NF-kB/ Rat分类
生物科学引用本文复制引用
许光勇,苏敬良,姜金奇,陈德龙,任晓明..乳酸调控大鼠肠黏膜微血管内皮细胞的NF-κB信号通路[J].中国实验动物学报,2012,20(4):80-86,7.基金项目
北京市自然科学基金(6072007) （6072007）
北京市教委资助项目(KM200910020002) （KM200910020002）
