中国比较医学杂志2012，Vol.22Issue(7)：5-8,4.DOI:10.3969.j.issn.1671.7856.2012.007.002
Ⅰ型牛疱疹病毒TK-/gE-基因双缺失突变株生物学特性
Study on the Characteristics of BHV-1 TK-/gE-/EGFP+ Gene-deleted Mutant Virus Strain
摘要
Abstract
Objective To detect the function of I BHV-1 TKVgE-/ EGFP+ gene-deleted mutant vims strain, find a new method to prevent infectious bovine rhinotracheitis (IBR) , the charaction of TK-/gE-/ EGFP+ gene-deleted mutant virus strain was further studied. Methods Southern hybridization, western dot blot and Plaques test were employed in this investment. Results TK gene is deleted and the mutant cannot produce glycoprotein E when gE gene is deleted. The diameter of mutant strain much smaller than that of field strain, the mutant strain grows on MDBK cells and the virus titer can keep 106. OTCID50/0. 1 mL for more than 40 hours, which indicated that the mutant strain inherit instantly. Conclusion The gene-deleted mutant virus strain was successful constructed in this study, and the mutant virus strain was stable. This will be useful for the further study of IBR.关键词
牛疱疹病毒/TK-/gE-突变株/生物学特性Key words
Bovine hebetes virus/ TK- /gE- gene-deleted mutant virus strain/ Biological characteristic分类
医药卫生引用本文复制引用
定明,范强,时彦胜,张小飞,张广州,李春,白杰英,刘正飞..Ⅰ型牛疱疹病毒TK-/gE-基因双缺失突变株生物学特性[J].中国比较医学杂志,2012,22(7):5-8,4.基金项目
农业公益性行业科研专项资助项目(200803018). （200803018）
