中国比较医学杂志2012，Vol.22Issue(7)：21-24,4.DOI:10.3969.j.issn.1671.7856.2012.007.006
猕猴B病毒gC蛋白特异性抗原表位的合成和表达
Synthesis and Expression of Monkey B Virus Glycoprotein C Specific Antigen Epitope
摘要
Abstract
Objective To get specific antigen epitope of B virus gC protein. Methods Synthesis specific antigen epitope of B virus gC gene by using synthesis gene of Long fragment, cloned the gene into the pMAL-5x vector and transformed into E. coli BL21 to express and purify the expression product. Results We get the B virus gC protein specific antigen protein successfully, and the protein expression in soluble form. Conclusion Prokaryotie expression system can produce soluble B virus gC protein antigen and can be used as the detection of B virus antigen关键词
猕猴B病毒/C蛋白/抗原表位/合成与表达Key words
Macaque B Virus/ gC protein/ Antigen epitope/ Synthesis and expression分类
生物科学引用本文复制引用
隋丽华,孙兆增,刘一,张小飞,崔晓霞,赵彦斌,刘冰,许琴,曾林..猕猴B病毒gC蛋白特异性抗原表位的合成和表达[J].中国比较医学杂志,2012,22(7):21-24,4.基金项目
十二五重大专项“高致病病原动物模型研究”(2012ZX10004502). （2012ZX10004502）
