中国比较医学杂志2012，Vol.22Issue(8)：48-50,55,封4,5.DOI:10.3969.j.issn.1671.7856.2012.008.011
ICR胎鼠成纤维细胞培养方法的优化
Optimization of the Culture Method of ICR Mouse Embryonic Fibroblasts
摘要
Abstract
Objective To investigate a most suitable method for harvesting mouse embryonic fibroblasts ( MEF) ). Methods Three different protocols including tissue only, cell-trypsin digestion, and tissue-trypsin digestion were applied for harvesting and culturing MEF. The MEF growth speed and cell morphology were also compared and analyzed respectively among the 3 methods. Results The cell proliferation was slow, cell size was small, but cells of other types were very few in the tissue only method, but the duration of purification was short. In the cell-trypsin digestion method, the cell proliferation was fast, cell size was somewhat large, but there were a little bit more cells of other types and the duration of purification was somehow longer. Finally, in the tissue-trypsin digestion method, the cell proliferation was very slow, cell size was very small, but there were also very few cells of other types. The duration of purification was very short. Conclusions Our findings indicate that the most efficient method for collecting and culturing mouse embryonic fibroblasts is the protocol of tissue-trypsin digestion.关键词
ICR小鼠/胚胎成纤维细胞/培养Key words
ICR mouse/ Embryonic fibroblast/ Culture分类
医药卫生引用本文复制引用
王英,刘石磊,刘振伟,刘云海,倪和民,邓桂馨,郭勇..ICR胎鼠成纤维细胞培养方法的优化[J].中国比较医学杂志,2012,22(8):48-50,55,封4,5.基金项目
国家自然基金面上资助项目(31072185,30871836),家禽产业技术体系北京市创新团队项目(2011,饲料营养功能). （31072185,30871836）
