中国动物传染病学报2012，Vol.20Issue(5)：22-26,5.
磷酸钙作为O型口蹄疫多肽疫苗佐剂的研究
CALCIUM PHOSPHATE AS ADJUVANT FOR TYPE O FOOD-AND-MOUTH DISEASE PEPTIDE VACCINE
摘要
Abstract
In order to explore the feasibility of calcium phosphate (CAP) as a vaccine adjuvant, the complex of CAP and type O Foot-and- mouth disease (FMD) peptide vaccine was prepare by co-precipitation. The rate of encapsulation efficiency was detected. Wistar rats were immunized intraperitoneally with the complex at day 0, 14 and 28. Tail vein blood samples were collected to detect FMD antibody levels. As a result, a certain amount of type O FMD peptide antigen was wrapped in CaP and the rate of encapsulation efficiency reached to 50%. The average antibody titer of the rats immunized with oil adjuvanted FMD antigen was 26 at day 14 and reached to 2^10 at day 42. However, the average antibody titer of the rats immunized with CaP adjuvanted FMD antigen was detectable only at day 21 post immunization and continued to rise to 27-28. The results show that CaP as an adjuvant improved immune response of type O foot-and- mouth disease peptide vaccine but its enhancement was lower than that ofoil adjuvant.关键词
磷酸钙/O型口蹄疫多肽/疫苗佐剂Key words
Calcium phosphate (CAP)/type O foot-and-mouth disease peptide/adjuvant分类
农业科技引用本文复制引用
郭沛,王霄旸,江善祥,薛飞群..磷酸钙作为O型口蹄疫多肽疫苗佐剂的研究[J].中国动物传染病学报,2012,20(5):22-26,5.基金项目
上海市科技兴农重点攻关项目【沪农科攻字（2009）第5-5号】 （）
