中国动物传染病学报2012，Vol.20Issue(5)：60-67,8.
猪细小病毒NS1基因密码子偏爱性分析
CODON PREFERENCE OF NONSTRUCTURAL PROTEIN NS1 GENE OF PORCINE PARVOVIRUS
摘要
Abstract
To provide a host system for optimal expression of major nonstructural protein (NS1) of Porcine parvovirus (PPV), codon preference was analyzed using CHIPS and CUSP programs of EMBOSS software. The results showed that CAI and ENC values of NS 1 gene were 0.642 and 39.703, indicating a strong bias in codon preference. Sixty-one codons in the predicted polypeptides were used with a strong bias towards A and T at the third codon position. There were obvious differences of 33, 26 and 27 codon frequencies between PPV NS 1 and E.coli, yeast and human, respectively. Furthermore, PPV NS 1 gene possessed total 71 rare codons that were located in E.coli and found serially strung together. The PPV NS1 was the most similar to yeast in codon usage patterns, suggesting that yeast should be preferentially used as the suitable expression system.关键词
猪细小病毒/NS1/密码子偏爱性Key words
Porcine parvovirus/nonstructural protein(NS)/codon preference分类
农业科技引用本文复制引用
罗丹丹,李兴玉,叶勇刚,王秋实,廖党金,李江凌,于吉锋,曹冶,谢晶..猪细小病毒NS1基因密码子偏爱性分析[J].中国动物传染病学报,2012,20(5):60-67,8.基金项目
四川生猪现代产业技术体系岗位专家 （）
四川省财政基础科研经费专项 （）
四川省繁育专项 （）
四川省畜牧科学研究院基本科研业务费专项【（SASA）2011A04】 （）
