中国兽医科学2012，Vol.42Issue(8)：825-830,6.
日本血吸虫抱雌沟蛋白DNA疫苗的研究
Studies on DNA vaccine encoding the full length of gynecophoral canal protein of Schistosoma japonicum
摘要
Abstract
To evaluate the protective efficiency and analyze immune response mechanism induced by vaccinating mice with the DNA vaccine of gynecophoral canal protein of Schistosoma japonicum（SjGCP）,the full length cDNA fragement of SjGCP was cloned into the eukaryotic expression vector pVAX1,with the recombinant eukaryotic expression plasmid pVAX1-SjGCP constructed.The purified recombinant plasmid pVAX1-SjGCP was applied to inject BALB/c mice intramuscularly for three times at the interval of two weeks.All the mice were challenged with 40±2 cercariae of S.japonicum after the third vaccination and sacrificed for perfusion on day 42 post-challenge.The worm burden reduction rate as well as egg reduction rate in liver and feces were evaluated.One week after last injection,the percentage of CD4＋ and CD8＋ lymphocyte subsets and the intracellular cytokines IL-4 and IFN-γ as well as the specific total IgG were detected to investigate the immunologic mechanism induced by the vaccination of DNA vaccine.In result,31.9% worm reduction and 47.85% and 68.04% of liver and feces egg reduction were obtained,respectively.The percentage of CD4＋,and CD8＋ lymphocytes and levels of IFN-γ cytokine and specific IgG antibody were significantly increased in mice vaccinated with pVAX1-SjGCP compared with those in pVAX1 injected control.In conclusion,the nucleic acid vaccine of Gynecophoral canal protein of S.japonicum could induce the responses of mixed Th1/Th2 cellular immunity and humoral immunity in animal against S.japonicum infection.关键词
日本血吸虫/抱雌沟蛋白/ORF/核酸疫苗/免疫机制Key words
Schistosoma japonicum/gynecophoral canal protein/full length cDNA/nucleic acid vaccine/immune response mechanism分类
农业科技引用本文复制引用
张娟,吕立婷,洪炀,傅志强,林矫矫..日本血吸虫抱雌沟蛋白DNA疫苗的研究[J].中国兽医科学,2012,42(8):825-830,6.基金项目
国家自然科学基金项目 （）
