中国兽医科学2012，Vol.42Issue(9)：916-920,5.
禽白血病病毒双抗体夹心ELISA检测方法的建立
Development of a double antibody sandwich ELISA method for the detection of avian leukosis virus
摘要
Abstract
A double antibody sandwich ELISA（DAS-ELISA） method was developed with monoclonal antibody against p27 of subgroup J avian leukosis virus（ALV） HB09JY03 as capture antibody and polyclonal antibody against the p27 as detector antibody to detect ALV rapidly and effectively.The sensitivity limit of the method was 1.25 ng/mL.No cross-reaction was observed with avian infectious bronchitis virus,Marek＇s disease virus,avian infectious laryngotracheitis virus and so on.It was suitable for detection of virus from the cloacal swabs of experimentally infected chicken at 12 day post-infection.A total of 491 albumin samples and 180 cloacal swabs were tested using the DAS-ELISA,and 96.5% and 93.8% agreement were found with the results of PCR,respectively.All of these results showed that the DAS-ELISA was a convenient,rapid and sensitive method for the detection of ALV,and the present study provided a tool for the early diagnosis of ALV and clarification of ALV in breeder chickens.关键词
禽白血病病毒/单克隆抗体/多克隆抗体/双抗体夹心ELISAKey words
avian leukosis virus/monoclonal antibody/polyclonal antibody/double antibody sandwich ELISA分类
农业科技引用本文复制引用
贠炳岭,刘文,李德龙,秦立廷,刘在斯,吴关,祁小乐,王永强,高宏雷,王笑梅,高玉龙..禽白血病病毒双抗体夹心ELISA检测方法的建立[J].中国兽医科学,2012,42(9):916-920,5.基金项目
公益性行业（农业）科研专项 （）
现代农业肉鸡产业技术体系建设项目 （）
哈尔滨市科技攻关计划项目 （）
