中国兽医科学2012，Vol.42Issue(9)：954-957,4.
犬新孢子虫不同靶基因PCR检测方法的比较
Comparison of different PCR methods for the detection of Neospora caninum target gene
摘要
Abstract
To find a PCR method which is more specific and sensitive to Neospora caninum,MAG1 gene,SAG1 gene and Nc5 gene of N.caninum were used as target genes and the sensitivity,specificity and detection rate of PCR of clinical samples were compared.The results showed that PCR method based on the Nc5 gene had the highest sensitivity,with minimum detectable amount as 17.8 fg/μL.While the MAG1 gene had the lowest sensitivity,with the minimum detectable amount of MAG1 gene as 17.8 pg/μL.The genomic DNA of Toxoplasma gondii,Theileria sergenti and Eperythrozoon suis could not be amplified by the three PCR methods,which indicated they had good specificity.Test on 28 tissue samples of BALB/c mouse that had been challenged by N.caninum standard strain showed that,75.0%（21/28） was identified by PCR based on the Nc5 gene,which was significantly higher than that of PCR based on the SAG1 gene 67.9%（19/28） and the MAG1 gene 53.6%（15/28）.This study provided a more sensitive and specific detection approach for neosporosis and epidemiological investigation.关键词
犬新孢子虫/MAG1基因/SAG1基因/Nc5基因/PCR方法Key words
Neospora caninum/MAG1 gene/SAG1 gene/Nc5 gene/PCR method分类
农业科技引用本文复制引用
郭焕平,贾立军,焦石,张守发..犬新孢子虫不同靶基因PCR检测方法的比较[J].中国兽医科学,2012,42(9):954-957,4.基金项目
国家自然科学基金资助项目 （）
吉林省自然科学基金项目 （）
