中国畜牧兽医2012，Vol.39Issue(9)：18-21,4.
马绒毛膜促性腺激素单克隆抗体的制备及鉴定
Preparation and Identification of Monoclonal Antibody Against Equine Chorionic Gonadotropin
摘要
Abstract
This study fusing the spleen cells of BALB/c mice which was immunized with high purified equine chorionic gon-adotropin and myeloma cells SP2/0 at the rate of 1 : 10, and screening the positive cloning by the means of indirect enzyme linked immunosorbent assay. Positive cells were clone through limited dilution method, injecting the mixture into the abdominal cavity of mice after intermediate cultured. Antibody titer was assayed by ways of indirect ELISA. Eight hybridoma cell lines were obtained which could secrete eCG stably, titer reached above 10-5. The obtained eCG hybridoma cell lines, which laid a foundation to assay the level of eCG.关键词
马绒毛膜促性腺激素/杂交瘤细胞株/单克隆抗体/酶联免疫吸附法Key words
equine chorionic gonadotropin/hybridoma cell line/monoclonal antibody/enzyme linked immunesorbent assay分类
医药卫生引用本文复制引用
苏晓倩,邓亮,贾瑜,韩国才..马绒毛膜促性腺激素单克隆抗体的制备及鉴定[J].中国畜牧兽医,2012,39(9):18-21,4.基金项目
国家公益性行业(农业)科研专项“马驴产业技术研究与试验示范”(201003075). （农业）
