中国畜牧兽医2012，Vol.39Issue(9)：195-202,8.
食品动物源产CTX-M-14大肠杆菌传播分子机制的演变
Evolutionally Dissemination of CTX-M-14 Producing Escherichia coli among Food-producing Animals
摘要
Abstract
Sixteen CTX-M-14 producing Escherichia coli isolates from food animal recovered from 2002 to 2009 were subjected to detection of extended spectrum beta-lactamases (ESBLs) encoding genes,plasmid mediated quinolone resistance determinants, and other relevant resistant genes (rmtB and floR) ; strains typing using PFGE and phylogrouping; blaCTX-M-14 plasmid replicon typing, transferring of blaCTX-M-14 by conjugation, and detection of context of blaCTX-M-14 gene. Majority of 16 isolates were clonally unrelated by PFGE,suggesting horizontally transfer of blaCTX-M-14 among food animals,and corresponded to phylogenetic groups A (50%) ,D (21. 5%) and Bl (28. 5%). Plasmids containing blaCTX-M-14. were belonging to IncK (n=3) ,IncHI2 (n = 1) ,IncHIl and IncN (n=2) ,IncFIB and IncF (n= 1) , IncF (n= 5) , or Incll (n= 1) , with insertion sequence of ISEcpl upstream blaCTX-M-14 ,and apparently increased in numbers of replicon and diversity from 2002 to 2009. The increasing dissemination of blaCTX-M-14 among different food-producing animals is mostly mediated by IncK, IncF,IncHI2, Incll plasmids carried by commensal E. coli. Some of CTX-M-14-producing E. coli harbored simultaneously blaCTX-M-79 or blaTEM-135 > as well other relevant resistant genes,such as oqxA, floR, aac(6 )-1b-cr or rmtB. BlaCTX-M-14 could be transferred separately in isolates of 2002, while co-transferred with other relevant resistant genes on the same plasmid via conjugation or transformation,suggesting co-selection and spread of CTX-M-14 producing isolates when use of any of the relevant antibiotics in animal farms.关键词
CTX-M-14/传播分子机制/复制子分型/水平传播Key words
CTX-M-14/ molecular mechanism of transmission/ replicon typing/ horizontal transmission分类
农业科技引用本文复制引用
甄盼盼,汤电,任艳娜,郭玉芳,王丽华,裘宗平,蒋红霞..食品动物源产CTX-M-14大肠杆菌传播分子机制的演变[J].中国畜牧兽医,2012,39(9):195-202,8.基金项目
国家自然科学基金—广东省自然科学基金联合基金重点项目(U1031004,U0631006). （U1031004,U0631006）
