中国药理学与毒理学杂志2012，Vol.26Issue(4)：546-549,4.DOI:10.3867/j.issn.1000-3002.2012.04.014
茶多酚二甲亚砜合剂对铀矿尘人支气管细胞毒性的防护作用
Protection of tea polyphenol and DMSO mixture against uranium dust-induced cytotoxicity in human bronchial epithelial cells
摘要
Abstract
OBJECTIVE To observe the uranium dust-induced DNA damage in BEAS-2B cells and the protective effect of tea polyphenol (TP) and DMSO mixture. METHODS Uranium dust, uranium + TP, uranium + DMSO, uranium+ TP+ DMSO were added into BEAS-2B cells for 24 h. Chromosomal damage was observed by micronucleus and multinuclear cells, DNA damage by comets assay, and expression of H2AX phosphorylated proteins and hypoxanthine phosporibosyl-transferase (HPRT) gene mutations were assayed by multinucleated cell method. RESULTS Compared with normal control group, the micronucleus rate, multinuclear rate, tailing rate, expression of H2AX protein phosphorylation and HPRT gene mutation rate in BEAS-2B cells were significantly increased (P＜0.05) after BEAS-2B cells were exposed to uranium dust. Uranium+TP, u-ranium+DMSO and uranium + TP + DMSO effectively reduced the changes ( P＜0. 05). Uranium + TP + DMSO mixture had the best protective effect. CONCLUSION Uranium dust can cause DNA damage in BEAS-2B cells. TP and DMSO mixture has a stronger protective effect.关键词
铀/茶多酚/二甲亚砜/DNA损伤Key words
uranium/tea polyphenol/dimethyl sulfoxide/DNA damage分类
医药卫生引用本文复制引用
黄波,龙颖,邱劲松,黄云,胡建安..茶多酚二甲亚砜合剂对铀矿尘人支气管细胞毒性的防护作用[J].中国药理学与毒理学杂志,2012,26(4):546-549,4.基金项目
国家自然科学基金(81072331),湖南省教育厅科研基金(07C619) （81072331）
