中国药业2012，Vol.21Issue(18)：24-25,2.
白花蛇舌草注射液诱导SKOV-3细胞株凋亡的研究
Study on Apoptosis Effect of Hedyotic Diffusa Willd Injection on SKOV-3 Cell Line
余萍1
作者信息
- 1. 重庆医疗器械质量检验中心·重庆市医疗器械质量与安全控制工程技术研究中心,重庆 401147
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摘要
Abstract
Objective To explore the influence and apoptosis ?inducing effect of Hedyotic Diffusa Willd Injection on the proliferation of human ovarian cancer cell line(SK0V-3) in vitro. Methods SKOV - 3 cells(5 x 104/mL) in logarithmic growth phase were respectively inoculated on the different cell culture plates. The test group was added 200 uX different concentrations of Hedyotic Diffusa Willd Injection and the control group was added isovolumetric PRMI - 1640 culture solution for incubation. The absorbance (Aim) was measured with the MTT colorimetric method and the inhibition rate of SKOV - 3 cells proliferation was calculated. After staining cells by Annexin - V - FITC/PI,the rates of cell apoptosis in the test group and the blank control group were detected by the flow cytometry and the morphological changes of apoptotic cells in different groups were investigated under fluorescent microscope. Results The detection Results of MTT method showed that the inhibition rate of SKOV - 3 cells proliferation was obviously amplified with the increase of concentration and action time of Hedyotic Diffusa Willd Injection. The inhibition rate in the test group with the concentration of 12 mL/L for 48 h culture was highest and reached (35. 67 ?. 16)%. The flow cytometry detection showed that Treating cells by Hedyotic Diffusa Willd Injection could cause the increasing trend of inhibition rate of SKOV - 3 cells proliferation with the concentration increase. The classic cellular apoptosis change in the treated SKOV - 3 cells was observed under the fluorescence microscope. Conclusion Hedyotic Diffusa Willd Injection could induce apoptosis of SKOV - 3 cells in ovarian cancer.关键词
白花蛇舌草注射液/SKOV-3细胞/增殖/凋亡Key words
Hedyotic Diffusa Willd Injection/ SKOV - 3 cells/ proliferation/ apoptosis分类
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余萍..白花蛇舌草注射液诱导SKOV-3细胞株凋亡的研究[J].中国药业,2012,21(18):24-25,2.
