中国药业2012，Vol.21Issue(18)：30-32,3.
高效液相色谱法测定万古霉素的血药浓度
Determination of Serum Concentration of Vancomycin by HPLC
杨林 1殷永红 1吴文兵1
作者信息
- 1. 湖北省黄石市中心医院药剂科,湖北黄石 435000
- 折叠
摘要
Abstract
Objective To estsblish a HPLC method for the determination of serum mass concentration of vancomycin. Methods Vancomycin was analyzed by the RP - HPLC system with Hypersil Cis column(250 mmx4.6 mm, 10 u,m) and the mobile phase of ace-tonitrile - potassium dihydrogen phosphate (10 : 90). The flow rate was 0.8 mL/min and the UV detection wavelength was 236 nm. With 10% ZnSO4 as the precipitation serum albumin, the supernatant after centrifugation was taken for detection by direct sample injection. Results The average recovery rate of vancomycin in low,medium and high concentrations (1.50,30.82,150.45 mg/L) were 99. 33% ,98. 94% and 99. 70% .respectively. The inter-day and intra - day relative standard deviations (RSD) were less than 5%(n = 5). The minimum detectable concentration of serum vancomycin was 0.5 mg/L. The linear range was 2.0-150 mg/L. The regress equations was C =0. 061 08/1+0. 000 384 5, r = 0. 999 9. Conclusion This method shows to be sensitive, accurate, simple, rapid, and suitable for monitoring the serum concentration of vancomycin and its pharmacokinetic study.关键词
万古霉素/血药浓度/高效液相色谱法Key words
vancomycin/ serum concentration/ HPLC分类
医药卫生引用本文复制引用
杨林,殷永红,吴文兵..高效液相色谱法测定万古霉素的血药浓度[J].中国药业,2012,21(18):30-32,3.
